Folding and stability of the aquaglyceroporin GlpF: Implications for human aqua(glycero)porin diseases
نویسندگان
چکیده
منابع مشابه
Energetics of glycerol conduction through aquaglyceroporin GlpF.
Aquaglyceroporin GlpF selectively conducts water and linear polyalcohols, such as glycerol, across the inner membrane of Escherichia coli. We report steered molecular dynamics simulations of glycerol conduction through GlpF, in which external forces accelerate the transchannel conduction in a manner that preserves the intrinsic conduction mechanism. The simulations reveal channel-glycerol hydro...
متن کاملDiffusion of glycerol through Escherichia coli aquaglyceroporin GlpF.
The glycerol uptake facilitator, GlpF, a major intrinsic protein found in Escherichia coli, selectively conducts water and glycerol across the inner membrane. The free energy landscape characterizing the assisted transport of glycerol by this homotetrameric aquaglyceroporin has been explored by means of equilibrium molecular dynamics over a timescale spanning 0.12 micros. To overcome the free e...
متن کاملGlycerol modulates water permeation through Escherichia coli aquaglyceroporin GlpF.
Among aquaglyceroporins that transport both water and glycerol across the cell membrane, Escherichia coli glycerol uptake facilitator (GlpF) is the most thoroughly studied. However, one question remains: Does glycerol modulate water permeation? This study answers this fundamental question by determining the three-dimensional potential of mean force of glycerol along the permeation path through ...
متن کاملImplications of Aquaglyceroporin 7 in Energy Metabolism
The aquaglyceroporin AQP7 is a pore-forming transmembrane protein that facilitates the transport of glycerol across cell membranes. Glycerol is utilized both in carbohydrate and lipid metabolism. It is primarily stored in white adipose tissue as part of the triglyceride molecules. During states with increased lipolysis, such as fasting and diabetes, glycerol is released from adipose tissue and ...
متن کاملFree-energy landscape of glycerol permeation through aquaglyceroporin GlpF determined from steered molecular dynamics simulations.
The free-energy landscape of glycerol permeation through the aquaglyceroporin GlpF has been estimated in the literature by the nonequilibrium method of steered molecular dynamics (SMD) simulations and by the equilibrium method of adaptive biasing force (ABF) simulations. However, the ABF results qualitatively disagree with the SMD results that were based on the Jarzynski equality (JE) relating ...
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ژورنال
عنوان ژورنال: Biochimica et Biophysica Acta (BBA) - Biomembranes
سال: 2015
ISSN: 0005-2736
DOI: 10.1016/j.bbamem.2014.11.015