Focusing on unpolymerized actin.

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Focusing on unpolymerized actin

~ scinating feature of cell locomotion is that net actin polymerization and depolymerization occur simultaneously at different locations in the cytoplasm, while the total concentration of polymerized actin remains roughly constant. Net polymerization occurs primarily at the cell front and net depolymerization occurs, depending on cell type, throughout or at the rear of the lamella (Wang, 1985; ...

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Non-muscle cells contain 15-500 microM actin, a large fraction of which is unpolymerized. Thus, the concentration of unpolymerized actin is well above the critical concentration for polymerization in vitro (0.2 microM). This fraction of actin could be prevented from polymerization by being ADP bound (therefore less favored to polymerize) or by being ATP bound and sequestered by a protein such a...

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ژورنال

عنوان ژورنال: Journal of Cell Biology

سال: 1993

ISSN: 0021-9525,1540-8140

DOI: 10.1083/jcb.123.1.1