FMRFamide-like substances in the leech. III. Biochemical characterization and physiological effects
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FMRFamide-like substances in the leech. III. Biochemical characterization and physiological effects.
FMRFamide-like immunoreactivity has been previously localized to identified neurons in the CNS of the leech, Hirudo medicinalis (Kuhlman et al., 1985a). These leech antigens have been characterized biochemically by reverse-phase high-pressure liquid chromatography (HPLC) followed by radioimmunoassay (RIA). The majority of the FMRFamide-like immunoreactivity recovered by HPLC from extracts of le...
متن کاملFMRF-amide-like substances in the leech. I. Immunocytochemical localization.
FMRF-amide-like immunoreactivity (FLI) was localized to approximately 50 neurons in each segmental ganglion of the medicinal leech using immunocytochemical techniques. Although most of these neurons were iterated in each segmental ganglion, some were more restricted in their segmental distribution. The head and tail ganglia likewise contained numerous FMRF-amide-like immunoreactive cells. In ad...
متن کاملSegment-specific effects of FMRFamide on membrane properties of heart interneurons in the leech.
1. The effects of Phe-Met-Arg-Phe (FMRF)amide (10(-6) M) on membrane properties of heart interneurons in the third, fourth, and fifth segmental ganglia [HN(3), HN(4), and HN(5) cells, respectively] of the leech were studied using discontinuous current-clamp and single-electrode voltage-clamp techniques. FMRFamide was focally applied onto the soma of the cell under investigation. 2. Application ...
متن کاملFMRF-amide-like substances in the leech. II. Bioactivity on the heartbeat system.
In the preceding paper (Kuhlman, J. R., C. Li, and R. L. Calabrese (1985) J. Neurosci. 5: 2301-2309) FMRF-amide-like immunoreactivity was localized to a specific set of neurons in the leech. Three types of these neurons are involved in controlling the animal's heartbeat: HE motor neurons and HA modulatory neurons which directly innervate the hearts, and the swim-initiating interneurons (cells 2...
متن کاملThe FMRFamide-Like Peptide Family in Nematodes
In the three decades since the FMRFamide peptide was isolated from the mollusk Macrocallista nimbosa, structurally similar peptides sharing a C-terminal RFamide motif have been identified across the animal kingdom. FMRFamide-like peptides (FLPs) represent the largest known family of neuropeptides in invertebrates. In the phylum Nematoda, at least 32 flp-genes are classified, making the FLP syst...
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ژورنال
عنوان ژورنال: The Journal of Neuroscience
سال: 1987
ISSN: 0270-6474,1529-2401
DOI: 10.1523/jneurosci.07-02-00595.1987