Fluorophore-Assisted Click Chemistry through Copper(I) Complexation

In vivo targeting through click chemistry.

Targeting small molecules to diseased tissues as therapy or diagnosis is a significant challenge in drug delivery. Drug-eluting devices implanted during invasive surgery allow the controlled presentation of drugs at the disease site, but cannot be modified once the surgery is complete. We demonstrate that bioorthogonal click chemistry can be used to target circulating small molecules to hydroge...

Exploded view of higher order G-quadruplex structures through click-chemistry assisted single-molecule mechanical unfolding.

Due to the long-range nature of high-order interactions between distal components in a biomolecule, transition dynamics of tertiary structures is often too complex to profile using conventional methods. Inspired by the exploded view in mechanical drawing, here, we used laser tweezers to mechanically dissect high-order DNA structures into two constituting G-quadruplexes in the promoter of the hu...

The Self-Assembly of Gold Nanoparticles Through "Click" Chemistry

Click chemistry with DNAw

Click Chemistry background information

Especially biomolecule labeling requires reaction procedures that can be performed under physiological conditions (neutral pH, aqueous solution, ambient temperature) with low reactant concentrations to ensure non-toxic, low background labeling at reasonable time scales while still preserving biological function. Among the plethora of possible reactions only a few generally fit the necessary rea...