Fluorophore-assisted carbohydrate electrophoresis (FACE) of glycosaminoglycans
نویسندگان
چکیده
منابع مشابه
Optimization of fluorophore-assisted carbohydrate electrophoresis.
Fluorophore-assisted carbohydrate electrophoresis (FACE) is a simple and inexpensive method for separating saccharides. Oligosaccharides were tagged with the charged fluorophore 8-aminonaphthalene-1,3,6-trisulfonate (ANTS), and the reductive amination reactions were essentially complete after approximately 16 h under the given experimental conditions. Saccharide-ANTS adducts were then separated...
متن کاملApplication of fluorophore-assisted carbohydrate electrophoresis to analysis of disaccharides and oligosaccharides derived from glycosaminoglycans.
Various combinations of fluorescent dyes, polyacrylamide gels, and electrophoresis buffers were tested by fluorophore-assisted carbohydrate electrophoresis (FACE) for the purpose of analyzing sulfated and nonsulfated glycosaminoglycan (GAG) oligosaccharides in which disaccharides and low-molecular weight oligosaccharides were included. A nonionic fluorescent dye was found to be suitable for ana...
متن کاملAnalysis of starch structure using fluorophore-assisted carbohydrate electrophoresis.
The analysis of the fine structure of starches is important to the investigation of linkages between starch structure and function and to the investigation of the properties and roles of starch biosynthetic, modifying and degradation enzymes. Fluorophore-assisted carbohydrate electrophoresis has recently been introduced as a method for the analysis of the oligosaccharide populations released by...
متن کاملAnalysis of IgA1 O-glycans in IgA nephropathy by fluorophore-assisted carbohydrate electrophoresis.
Abnormal O-glycosylation of IgA1 may contribute to pathogenic mechanisms in IgA nephropathy (IgAN). Observations of altered lectin binding to IgA1 in IgAN suggest that the O-glycan chains may be undergalactosylated, but precise structural definition of the defect has proved technically difficult, and it remains unconfirmed. This is the first study using fluorophore-assisted carbohydrate electro...
متن کاملIsolation and analysis of sugar nucleotides using solid phase extraction and fluorophore assisted carbohydrate electrophoresis
The building blocks of simple and complex oligosaccharides, termed sugar nucleotides, are often overlooked for their role in metabolic diseases and may hold the key to the underlying disease pathogenesis. Multiple reasons may account for the lack of analysis and quantitation of these sugar nucleotides, including the difficulty in isolation and purification as well as the required expensive inst...
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ژورنال
عنوان ژورنال: Osteoarthritis and Cartilage
سال: 2001
ISSN: 1063-4584
DOI: 10.1053/joca.2001.0439