Fluorometric Assay of Sialic Acid in Brain Gangliosides
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چکیده
منابع مشابه
Fluorometric Assay of Sialic Acid in Brain Gangliosides.
In pursuing quantitative histochemical investigations aimed at demonstrating the biochemical architecture of human frontal cortex (l-4), we found it necessary to determine ganglioside sialic acid in submicrogram amounts. Even when adapted to the microscale, spectrophotometric methods lack the sensitivity required for studying nervous tissue samples containing less than 0.3 pg of sialic acid. Si...
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A method is described for analysis of gangliosides by GLC assay of the sialic acid component. Mild acid treatment in methanol converted the latter to methyl ketoside methyl ester, which was then chromatographed as the TMS derivative. The major methanolysis product was shown to be the beta-anomer, and its chromatographic peak was used for quantification. NANA and NGNA could be analyzed simultane...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1964
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)97707-0