Fluorogen activating protein toolset for protein trafficking measurements
نویسندگان
چکیده
منابع مشابه
Near-instant surface-selective fluorogenic protein quantification using sulfonated triarylmethane dyes and fluorogen activating proteins.
Agonist-promoted G-protein coupled receptor (GPCR) endocytosis and recycling plays an important role in many signaling events in the cell. However, the approaches that allow fast and quantitative analysis of such processes still remain limited. Here we report an improved labeling approach based on the genetic fusion of a fluorogen activating protein (FAP) to a GPCR and binding of a sulfonated a...
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ژورنال
عنوان ژورنال: Traffic
سال: 2020
ISSN: 1398-9219,1600-0854
DOI: 10.1111/tra.12722