Fluorescent staining of Neurospora nuclei with DAPI

Fluorescent staining of fungal nuclei with a benzimidazol derivative.

A direct staining procedure is described for fluorescence microscopy of fungal nuclei, chromosomes and mitochondria. The fluorochrome is a benzimidazol derivative (33258 Hoechst) known to bind selectively to deoxyribonucleic acid at neutral pH. The advantages of 33258 Hoechst relative to Feulgen compounds used previously to stain these structures include a greater intensity of fluorescence, th...

DAPI: a DNA-specific fluorescent probe.

DAPI (4',6-diamidino-2-phenylindole) is a DNA-specific probe which forms a fluorescent complex by attaching in the minor grove of A-T rich sequences of DNA. It also forms nonfluorescent intercalative complexes with double-stranded nucleic acids. The physicochemical properties of the dye and its complexes with nucleic acids and history of the development of this dye as a biological stain are des...

Use of DAPI staining for quantitation of cell chemotaxis.

Segmentation and shape classification of nuclei in DAPI images

This paper addresses issues of analysis of DAPI-stained microscopy images of cell samples, particularly classification of objects as single nuclei, nuclei clusters or nonnuclear material. First, segmentation is significantly improved compared to Otsu’s method [5] by choosing a more appropriate threshold, using a cost-function that explicitly relates to the quality of resulting boundary, rather ...

Fluorescent staining of Histoplasma capsulatum.

In an earlier paper from this laboratory (Gordon, 1958) it was demonstrated that fluorescein-labeled specific antiserum employed as a cellular stain will differentiate Candida albicans and Candida tropicalis from other yeasts and yeastlike organisms, including the remaining members of the genus Candida. The present report describes the preparation and use of a similar reagent for detection and ...