Fluorescent labeling of Acanthamoeba assessed in situ from corneal sectioned microscopy
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چکیده
منابع مشابه
Fluorescent labeling of Acanthamoeba assessed in situ from corneal sectioned microscopy
Acanthamoeba keratitis is a serious pathogenic corneal disease, with challenging diagnosis. Standard diagnostic methods include corneal biopsy (involving cell culture) and in vivo reflection corneal microscopy (in which the visualization of the pathogen is challenged by the presence of multiple reflectance corneal structures). We present a new imaging method based on fluorescence sectioned micr...
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BACKGROUND Corneal nerves can be examined using in vivo confocal microscopy (IVCM). This new technique permits sequential observation of the corneal subbasal nerve plexus and detects early signs of diabetic peripheral neuropathy. OBJECTIVE To describe a patient with autoimmune peripheral neuropathy followed up using corneal IVCM. DESIGN Case report. SETTING Clinic of neurology, Geneva, Sw...
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Tremendous progress in recent computer-controlled systems for fluorescence and laser-confocal microscopy has provided us with powerful tools to visualize and analyze molecular events in the cells. Various fluorescent staining and labeling techniques have also been developed to be used with these powerful instruments. Fluorescent proteins such as green fluorescent protein (GFP) allow us to direc...
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Science, founded in 1880, is published each Friday by the American Association for the Advancement of Science at the Business Press, 10 McGovern Ave., Lancaster, Pa. Entered as second-class matter at the Post Office at Lancaster, Pa., January 13, 1948, under the Act of March 3, 1879. Acceptance for mailing at the special rate postage provided for in the Act of February 28, 1925, embodied in Par...
متن کاملThe relative value of confocal microscopy and superficial corneal scrapings in the diagnosis of Acanthamoeba keratitis.
PURPOSE To compare the relative diagnostic value of confocal microscopy and superficial corneal cultures in the diagnosis of Acanthamoeba keratitis by using clinical and microbiologic definitions of disease. METHODS Results of confocal microscopy, superficial corneal smear, and superficial corneal culture were analyzed for validity against 2 different microbiologic and a clinical composite st...
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ژورنال
عنوان ژورنال: Biomedical Optics Express
سال: 2012
ISSN: 2156-7085,2156-7085
DOI: 10.1364/boe.3.002489