منابع مشابه
Crystal Structure and Spectroscopic Characterization of a Luminescent Europium Chelate
We report the crystal structure at 1 Å resolution of europium bound to diethylenetriaminepentaacetate covalently coupled to an organic chromophore, carbostyril 124 (DTPA-cs124; carbostyril 124 ) 7-amino-4-methyl-2(1H)quinolinone). The complex is highly luminescent in solution and in the crystal form. The Eu-DTPA-cs124 crystallizes in the space group C2/c with two molecules in the asymmetric uni...
متن کاملCell assay using a two-photon-excited europium chelate
We report application of two-photon excitation of europium chelates to immunolabeling of epidermal growth factor receptor (EGFR) cell surface proteins on A431 cancer cells. The europium chelates are excited with two photons of infrared light and emit in the visible. Europium chelates are conjugated to antibodies for EGFR. A431 (human epidermoid carcinoma) cells are labeled with this conjugate a...
متن کاملTime-Resolved Luminescence Microscopy and Microarray Using Europium Chelate Labels
The luminescent Eu complex, DTBTA-Eu, is highly stable in terms of ligand-metal dissociation, and can be labelled to amino groups of proteins and other bio-molecules. Owing to the long lifetime of Eu luminescence, time-resolved measurement of DTBTA-Eu removes background fluorescence and enhances the sensitivity of the labelled materials. This property was applied to time-resolved luminescence m...
متن کاملVery bright europium complexes that stain cellular mitochondria.
The synthesis, structure and photophysical properties of a series of highly emissive europium complexes is reported. Certain complexes enter mammalian cells by macropinocytosis and stain the mitochondria selectively, allowing observation of the Eu emission in cellulo by time-gated spectral imaging.
متن کاملFluorescent probes that stain living nerve terminals.
We have evaluated the efficacy of 18 cationic mitochondrial dyes that, as a class, show some ability to stain living nerve terminals. Several of these agents provide excellent staining of neuromuscular junctions in a wide range of species. More detailed studies of the most effective of these dyes--4-(4-diethylaminostyryl)-N-methylpyridinium iodide (4-Di-2-ASP)--indicate that it has no lasting e...
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ژورنال
عنوان ژورنال: Journal of Bacteriology
سال: 1969
ISSN: 0021-9193,1098-5530
DOI: 10.1128/jb.98.1.246-248.1969