FLUORESCENT ANTIBODY STAINING OF PR8 INFLUENZA VIRUS ADSORBED ON CHICKEN ERYTHROCYTES

Fluorescent antibody staining of PR8 influenza virus adsorbed on chicken erythrocytes.

nolphthalein diphosphate agar (Barber and Kuper, J. Pathol. Bacteriol. 63:65, 1951); and (iv) fluorescein amine agar. As a fermentation medium, mannitol broth showed significantly greater agreement with the coagulase test than did mannitol salt agar. The following medium proved excellent in differentiating pigment production in staphylococci: Tryptose agar (Difco) was dissolved according to pac...

Electrophoretic Studies on Pr8 Influenza Virus

Crude preparations of PR8 influenza virus, obtained by high-speed centrifugation, contain two electrophoretically distinct components. One of these, present to the extent of 10 to 20 per cent, was identified by electrophoresis, centrifuge, and activity tests, as an impurity similar to or identical with a high molecular weight acidic substance shown by Knight to be elaborated by normal uninfecte...

Studies on Fluorescent Antibody Staining

1. A study has been made of the non-specific fluorescent staining of splenic imprints treated with fluorescent sheep antibody globulins. 2. In tissue imprints made with the spleens of antigen-stimulated animals, no morphological distinction was evident between areas showing non-specific fluorescence and specific fluorescence. 3. Elimination of non-specific fluorescence was not achieved by any o...

The Sorption of Influenza Virus by Chicken Erythrocytes

Data are presented which indicate that the forces of attraction between influenza virus and chicken erythrocytes are governed by an orderly mechanism which effects a proportional distribution of virus between erythrocytes and suspending fluid. For a unit mass of erythrocytes, the ratio of combined (sorbed) virus to free (unsorbed) virus is sufficiently constant over a wide range of virus concen...

Visualization of Horse Sickness virus by the Fluorescent antibody technique