منابع مشابه
Fluorescence and fluorescence microscopy
The concept of specific cellular antigen staining by use of an immunofluorescent technique was introduced by AH COONS and co-workers about 60 years ago. Fluorescent probes are efficient tools and enable the detection of particular components in complex structures of organs including live cells. Under the condition of specific molecular interactions, fluorochrome labeled ligands allow the select...
متن کاملFluorescence Microscopy
When organic or inorganic specimens absorb and subsequently reradiate light, the process is typically a result of fluorescence or phosphorescence. Fluorescence emission is nearly simultaneous with the absorption of the excitation light as the time delay between photon absorption and emission is typically less than a microsecond. When the emission persists long after the excitation light is exti...
متن کاملFluorescence Microscopy
The human eye requires contrast to perceive details of objects. Several ingenious methods of improving the contrast of microscopy images have been designed, and each of them opened new applications of optical microscopy in biology. The simplest and very effective contrasting method is ‘‘dark field.’’ It exploits the scattering of light on small particles that differ from their environment in re...
متن کاملQuantitative Fluorescence Microscopy
Printed in the Netherlands " You have walked to the edge of the great mosaic of human knowledge. Up until now, you have been living in a world full of ideas and concepts that other people have set out for you. Now it is your turn. You get to design a piece of the mosaic and glue it down. It just has to fit with what else is there. And if you do a good job shaping your tile, it will be easier fo...
متن کاملSpectral imaging fluorescence microscopy.
The spectral resolution of fluorescence microscope images in living cells is achieved by using a confocal laser scanning microscope equipped with grating optics. This capability of temporal and spectral resolution is especially useful for detecting spectral changes of a fluorescent dye; for example, those associated with fluorescence resonance energy transfer (FRET). Using the spectral imaging ...
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ژورنال
عنوان ژورنال: Annals of the New York Academy of Sciences
سال: 1971
ISSN: 0077-8923,1749-6632
DOI: 10.1111/j.1749-6632.1971.tb35068.x