منابع مشابه
Fluorescein-conjugated Bovine Albumin
Fluorescein-bovine albumin conjugates have been prepared and found not to differ appreciably in size, shape, and homogeneity from the precursor, bovine serum albumin. Fluorescein has also been conjugated to rat plasma proteins. Their disappearance rates from the circulation of rats correspond with those obtained from the use of isotope labeling. Their sites of localization in rat tissues were s...
متن کاملEffect of the labelling ratio on the photophysics of fluorescein isothiocyanate (FITC) conjugated to bovine serum albumin.
The non-linearity of the fluorescence emission on increasing the probe to protein ratio has long been regarded as problematic and has lead to the development of dyes to overcome this effect. One of the causes of this non-linear response can be ascribed to the overlap of the label's own absorption and emission spectra. At higher labelling ratios, this affords the possibility of a reasonably effi...
متن کاملThiol-reactive clenbuterol analogues conjugated to bovine serum albumin.
Several novel thiol-reactive clenbuterol analogues were coupled in high yield with bovine serum albumin (BSA). After labelling of unreacted cysteines with maleimide spin label (MiSL), the yield of the coupling reaction was determined by electron paramagnetic resonance (EPR) spectroscopy and spectral analysis. Two spin-probe populations with different mobility states were quantitatively determin...
متن کاملFluorescence dynamics of bovine serum albumin (BSA) conjugated CdZnS nanocrystallites
We report on the production of composite semiconductor CdZnS nanoparticles by adopting an inverse micellar route, using bis (2-ethylhexyl) sulfosuccinate (aerosol-AOT) as surfactant and with a degree of hydration w0 = [H2O] : [AOT] = 8.9. Prior to bioconjugation (conjugation with bovine serum albumin (BSA)), the hydrophobic surface of the nanocrystals were made hydrophilic with thiol treatment ...
متن کاملPolyclonal antibody production against bovine serum albumin conjugated artemisinin in rabbit
Abstract: The aim of the present study was to produce a polyclonal antibody against bovine serum albumin (BSA) conjugated with artemisinin. To gain an immunogenic character of artemisinin, a carboxyl group was added to it using mixed anhydride method. Then, the reactive compound of artemisinin was conjugated with BSA. The BSA+artemisinin were injected to white female New Zealand rabbits for two...
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ژورنال
عنوان ژورنال: Journal of General Physiology
سال: 1953
ISSN: 1540-7748,0022-1295
DOI: 10.1085/jgp.36.4.489