منابع مشابه
Gene Activation Using FLP Recombinase in C. elegans
The FLP enzyme catalyzes recombination between specific target sequences in DNA. Here we use FLP to temporally and spatially control gene expression in the nematode C. elegans. Transcription is blocked by the presence of an "off cassette" between the promoter and the coding region of the desired product. The "off cassette" is composed of a transcriptional terminator flanked by FLP recognition t...
متن کاملFLP Recombinase-Mediated Site-Specific Recombination in Silkworm, Bombyx mori
A comprehensive understanding of gene function and the production of site-specific genetically modified mutants are two major goals of genetic engineering in the post-genomic era. Although site-specific recombination systems have been powerful tools for genome manipulation of many organisms, they have not yet been established for use in the manipulation of the silkworm Bombyx mori genome. In th...
متن کاملFlp recombinase regulated lacZ expression at the ROSA26 locus.
Manipulation of gene expression patterns through gene targeting in mouse embryonic stem (ES) cells (Thomas and Capecchi, 1987) and the more recent advent of conditional mutagenesis has revolutionized mouse genetics. The recombinase system most commonly used for conditional alleles in mouse is Cre/loxP derived from bacteriophage P1 (Sauer and Henderson, 1988). While less utilized, FLP/FRT derive...
متن کاملDNA recognition by the FLP recombinase of the yeast 2 mu plasmid. A mutational analysis of the FLP binding site.
The 2 mu plasmid of the yeast Saccharomyces cerevisiae encodes a site-specific recombination system consisting of the FLP protein and two inverted recombination sites on the plasmid. The minimal fully functional substrate for in-vitro recombination in this system consists of two FLP protein binding sites separated by an eight base-pair spacer sequence. We have used site-directed mutagenesis to ...
متن کاملStepwise manipulation of DNA specificity in Flp recombinase: progressively adapting Flp to individual and combinatorial mutations in its target site.
The Flp protein from Saccharomyces cerevisiae is one of the site-specific tyrosine family recombinases that are used widely in genomic engineering. As a first step towards mediating directed DNA rearrangements at non-native Flp recombination targets (mFRTs), we have evolved three separate groups of Flp variants that preferentially act on mFRTs containing substitutions at the first, seventh or b...
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ژورنال
عنوان ژورنال: Proceedings of the National Academy of Sciences
سال: 1988
ISSN: 0027-8424,1091-6490
DOI: 10.1073/pnas.85.13.4628