Flow Cytometric Sorting of Paraffin-Embedded Tumor Tissues Considerably Improves Molecular Genetic Analysis
نویسندگان
چکیده
منابع مشابه
Flow cytometric sorting of paraffin-embedded tumor tissues considerably improves molecular genetic analysis.
The characterization of genetic aberrations in paraffin-embedded tumor material is impaired by contaminating normal cells. In the present study on the genetic causes of loss of HLA expression in diffuse large B-cell lymphoma (DLBCL), we compared the efficacy of microdissection with flow cytometric sorting of tumor cells. Single-cell suspensions from paraffin-embedded material of 5 DLBCL cases w...
متن کاملFlow Cytometric DNA Analysis and Histopathologic Re-Evaluation of Paraffin Embedded Samples from Hydatidiform Moles and Hydropic Abortions
Background Distinction of hydatidiform moles (HMs) from non-molar abortions and sub-classification of HMs are important for clinical practice; yet, diagnosis based solely on morphology is affected by interobserver variability. The objective of this study was to determine the role of DNA flow cytometry in distinguishing molar from non-molar pregnancies. MaterialsAndMethods This retrospective stu...
متن کاملProteomic analysis of RCL2 paraffin-embedded tissues
Histopathological diagnosis in most of the world's hospitals is based upon formalin-fixed and paraffin-embedded (FFPE) tissues. Although this standard fixation and embedding procedure keeps the tissue in excellent form for morphological and immunohistological analysis, FFPE is inappropriate for nucleic acids and protein studies. We investigated the potential value of RCL2, a new non-toxic fixat...
متن کاملflow cytometric dna analysis and histopathologic re-evaluation of paraffin embedded samples from hydatidiform moles and hydropic abortions
background: distinction of hydatidiform moles (hms) from non-molar abortions and sub-classification of hms are important for clinical practice; yet, diagnosis based solely on morphology is affected by interobserver variability. the objective of this study was to determine the role of dna flow cytometry in distinguishing molar from non-molar pregnancies. materials and methods: this retrospective...
متن کاملMolecular detection and genotypic characterization of Toxoplasma gondii in paraffin-embedded fetoplacental tissues of women with recurrent spontaneous abortion
Background Congenital toxoplasmosis is an important cause of spontaneous abortion worldwide. However, there is limited information on detection and genotypic characterization of T. gondii in women with recurrent spontaneous abortion (RSA). The aim of this study is molecular detection and genotypic characterization of T. gondii in formalin-fixed, paraffin-embedded fetoplacental tissues (FFPTs) o...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: American Journal of Clinical Pathology
سال: 2003
ISSN: 0002-9173,1943-7722
DOI: 10.1309/hpr11r7lq9nnccg8