Flow Cytometric Eosin-5′-Maleimide Test is a Sensitive Screen for Hereditary Spherocytosis
نویسندگان
چکیده
منابع مشابه
Comparison study of the eosin-5'-maleimide binding test, flow cytometric osmotic fragility test, and cryohemolysis test in the diagnosis of hereditary spherocytosis.
OBJECTIVES Current guidelines recommend the eosin-5'-maleimide (EMA) binding test and cryohemolysis test for screening for hereditary spherocytosis (HS), and the flow cytometric osmotic fragility (FC OF) test was recently developed to replace the classic OF test. We evaluatedthe performance of the EMA binding test, FC OF test, cryohemolysis test, and the hemoglobin (Hb)/mean corpuscular hemoglo...
متن کاملRapid flow cytometric test using eosin-5-maleimide for diagnosis of red blood cell membrane disorders.
Conventional diagnosis of hereditary red blood cell (RBC) membrane disorders, in particular hereditary spherocytosis (HS), is labor intensive, time consuming and requires at least 2 ml of blood, which might be impractical in neonatal period. We evaluated the use of eosin-5-maleimide (EMA), a dye that reacts covalently with lysine-430 on the first extracellular loop of band 3 protein, for rapid ...
متن کاملStability of eosin-5'-maleimide dye used in flow cytometric analysis for red cell membrane disorders
BACKGROUND The eosin-5'-maleimide (EMA) binding test using flow cytometry is a common method to measure reduced mean channel fluorescence (MCF) of EMA-labeled red blood cells (RBCs) from patients with red cell membrane disorders. The basic principle of the EMA-RBC binding test involves the covalent binding of EMA to lysine-430 on the first extracellular loop of band 3 protein. METHODS In the ...
متن کاملFlow cytometry test for hereditary spherocytosis.
We read with interest the recent article of Bianchi et al. published in Haematologica that compared laboratory tests for hereditary spherocytosis (HS) in 150 patients. The authors reported the usefulness of the eosine-5maleimide binding (EMA) test in the diagnosis of HS with high sensitivity and specificity, and confirmed, as we had previously reported, that this test was independent of the typ...
متن کاملAnalysis of Hereditary Elliptocytosis with Decreased Binding of Eosin-5-maleimide to Red Blood Cells
Flow cytometric test for analyzing the eosin-5-maleimide (EMA) binding to red blood cells has been believed to be a specific method for diagnosing hereditary spherocytosis (HS). However, it has been reported that diseases other than HS, such as hereditary pyropoikilocytosis (HPP) and Southeast Asian ovalocytosis (SAO), which are forms in the category of hereditary elliptocytosis (HE), show decr...
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ژورنال
عنوان ژورنال: Indian Journal of Hematology and Blood Transfusion
سال: 2017
ISSN: 0971-4502,0974-0449
DOI: 10.1007/s12288-017-0907-8