Flexible antibodies with nonprotein hinges

Flexible antibodies with nonprotein hinges

There is a significant need for antibodies that can bind targets with greater affinity. Here we describe a novel strategy employing chemical semisynthesis to produce symmetroadhesins: antibody-like molecules having nonprotein hinge regions that are more flexible and extendible and are capable of two-handed binding. Native chemical ligation was carried out under mild, non-denaturing conditions t...

Flexible Hinges in Bacterial Chemoreceptors.

Transmembrane bacterial chemoreceptors are extended, rod-shaped homodimers with ligand-binding sites at one end and interaction sites for signaling complex formation and histidine kinase control at the other. There are atomic-resolution structures of chemoreceptor fragments but not of intact, membrane-inserted receptors. Electron tomography of in vivo signaling complex arrays lack distinct dens...

Elastica with Hinges

Parallel-plate electrostatic actuation with vertical hinges

A microactuating structure with vertical polyimide hinges has been designed, fabricated and tested. Unlike traditional microactuators, which are located on the substrate surface, this new actuation mechanism is capable of producing linear motion at a point far from the substrate. This advantage can make it possible to drive other out-of-plane folded structures for on-wafer or out-of-wafer appli...

Dual architectural roles of HU: formation of flexible hinges and rigid filaments.

The nucleoid-associated protein HU is one of the most abundant proteins in Escherichia coli and has been suggested to play an important role in bacterial nucleoid organization and regulation. Although the regulatory aspects of HU have been firmly established, much less is understood about the role of HU in shaping the bacterial nucleoid. In both functions (local) modulation of DNA architecture ...