Flavin Mononucleotide: The Coenzyme of Reduced Diphosphopyridine Nucleotide Dehydrogenase
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منابع مشابه
Flavin mononucleotide: the coenzyme of reduced diphosphopyridine nucleotide dehydrogenase.
From the particulate reduced nicotinamide adenine dinucleotide (DPNH) oxidase of beef heart, Mackler (3) has obtained a soluble DPNH dehydrogenase which was estimated to be approximately 90 to 95% homogenous when examined electrophoretically.l Similarly, when the enzyme was chromatographed on diethylaminoethyl cellulose with phosphate buffer, pH 7.5, in a gradient elution method (0.001 M to 0.0...
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We have shown in a previous communication (1) that the enzyme triosephosphate dehydrogenase is rapidly inactivated in the presence of reduced diphosphopyridine nucleotide at physiological concentrations, temperature, and pH. Good stoichiometry was shown for the amount of enzyme inactivation, DPNH-X1 formation, and disappearance of sulfhydryl groups. It was then of particular interest to determi...
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3. One mole of the apoenzyme, molecular weight 36,000, will bind 1 mole of flavin mononucleotide, 2 moles of flavin adenine dinucleotide, or 1 of each when presented with them at equal concentrations. Two flavin-binding sites are present; one is common to both flavins and is catalytically active. The other is specific for flavin adenine dinucleotide and is not involved in catalysis. Equal conce...
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In continuation of our investigation of liver diphosphopyridine nucleotide-linked aldehyde dehydrogenase (1)) we have undertaken a study of various classes of inhibitors of the enzyme. Interest in aldehyde oxidation has been increasing in recent years from several points of view. The classical problem of ethanol oxidation in viva still has many unresolved facets (Z), and the enzymes responsible...
متن کاملFlavin Mononucleotide: the Acid-extractable Flavin of the Dpnh-coenzyme Q Reductase of the Respiratory Chain.
Several D P N H dehydrogenases have been purified from heart mitochondria or heart-muscle preparationsl-L STRAUB'S enzym& has been shown by MASSEY 8 to be a form of lipoamide dehydrogenase (EC 1.6.4.3), while the relationship of the remaining preparations to the respiratory chain and to each other is obscure. The flavin extractable by acid t reatment has been reported to be FMN in some casesl, ...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1963
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(19)84018-8