Fixation Process With 10% KOH Immersion And Variation Of Heating Temperatures On The Quality Of Pediculus humanus capitis

The preparation of preserved preparations is one the useful methods to determine morphology ectoparasites and insects whose manufacturing process goes through four stages starting with 10% KOH fixation, dehydration process, clearing mounting process. immersion serves thin out chitin layer that forms exoskeleton, which a thick covering on body surface Pediculus humanus capitis (human lice). purpose this study was quality by immersing them in at temperatures 60°C, 70°C, 80°C, 90°C for 30 minutes using whole mount method, order observe entire species clearly. sample used adult stage, as many 24 tails. Observation assessed based clarity, color quality, morphological integrity. At temperature two good qualities are obtained. Three obtained 70oC. it 90°C, there no quality. results were then subjected statistical tests Kruskall-Wallis test, p value 0.040<0.05, so could be interpreted an influence variations heating preparations. capitis. namely, 70°C minutes, complete characteristics, transparent color, clear appearance.