Five Penicillin-binding Components Occur in Bacillus subtilis Membranes
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چکیده
منابع مشابه
Five penicillin-binding components occur in Bacillus subtilis membranes.
Membranes of BaciZZus subtilis to which [14C]penicillin G had been bound were fractionated by polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate. Five labeled components were separated. These five components differed from each other in the rates at which they bound penicillins and cephalosporins by up to four orders of magnitude. The major penicillin-binding component,...
متن کاملIsolation by covalent affinity chromatography of the penicillin-binding components from membranes of Bacillus subtilis.
An affinity chromatography technique was developed to isolate the five penicillin-binding components present in Bacillus subtilis membranes. The proteins were solubilized by the detergent Nonidet P-40, bound covalently to penicillin-substituted Sepharose, and subsequently eluted from the matrix with neutral hydroxylamine, which cleaves the penicilloyl-enzyme bond. Penicillin binding-component V...
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Penicillin- (cloxacillin-) resistant mutants of Bacillus subtilis were isolated in a stepwise fashion and the five penicillin-binding components (PBCs) in each were examined to determine which of the proteins, if any, corresponds to the penicillin killing site. PBCs II and V were previously eliminated as the likely penicillin targett. In the present work, PBC IV showed no change in sensitivity ...
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A variety of techniques have been employed to investigate the penicillin-binding components of Bacillus subfilis, Bacillus cereus, Staphylococcus aureus, and Escherichia coli. Virtually all the binding sites in these organisms are located in the cell membrane. All the techniques which have been employed suggest that more than one binding component exists in each of these organisms, although S. ...
متن کاملMultiple penicillin-binding components in Bacillus subtilis, Bacillus cereus, Staphylococcus aureus, and Escherichia coli.
A variety of techniques have been employed to investigate the penicillin-binding components of Bacillus subfilis, Bacillus cereus, Staphylococcus aureus, and Escherichia coli. Virtually all the binding sites in these organisms are located in the cell membrane. All the techniques which have been employed suggest that more than one binding component exists in each of these organisms, although S. ...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1972
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(20)81815-8