First study of sperm mediated gene transfer in Egyptian river buffalo
نویسندگان
چکیده
منابع مشابه
P-85: Epididymal Sperm Parameters following Sperm Mediated Gene Transfer of EDFP-pIRES Vector in Rat
Background: Sperm mediated gene transfer was approved for producing transgenic animals. There are many studies which reported different success rate by this procedure. Failure in this project mostly is returned to transfection and stability of transferred genes. The other problem that may contribute in project failure may return to the testicular damages which occur at the time of transfection,...
متن کاملSperm-mediated gene transfer.
Since 1989, a new method for the production of transgenic animals has been available, namely sperm-mediated gene transfer (SMGT), based on the intrinsic ability of sperm cells to bind and internalise exogenous DNA molecules and to transfer them into the oocyte at fertilisation. We first described the SMGT procedure in a small animal model, with high efficiency reported in the mouse. In addition...
متن کاملSperm Mediated Gene Transfer (SMGT) in Chicken
A transgenic animal as originally defined by Gordon and Ruddle (1981), is an organism carrying recombinant molecules that were intentionally introduced by human intervention. Classically this entailed the stable integration of the transgene within the host animal’s genome and its transmission to progeny through normal breeding programmes. Transgenic chicken holds great promise in basic biologic...
متن کاملSperm-mediated gene transfer: applications and implications.
Recent developments in studies of sperm-mediated gene transfer (SMGT) now provide solid ground for the notion that sperm cells can act as vectors for exogenous genetic sequences. A substantive body of evidence indicates that SMGT is potentially useable in animal transgenesis, but also suggests that the final fate of the exogenous sequences transferred by sperm is not always predictable. The ana...
متن کاملO-10: Sperm Mediated Gene Transfer Using Adjuvant Preserving Fertility for Production of Transgenic Chicken Expressing
Background: Low uptake of exogenous DNA by sperm and reduced number of fertilized oocyte by transfected sperm are the major obstacles for progression of sperm mediated gene transfer. Therefore, the modification of sperm mediated gene transfer procedure needs to be required. The purpose of this study was to evaluate the efficiency of FuGene 6 compare to lipofection in transfection medium for int...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Genetic Engineering and Biotechnology
سال: 2017
ISSN: 1687-157X
DOI: 10.1016/j.jgeb.2017.06.003