First Report of Prunus Necrotic Ringspot Virus Infecting Red Mulberry (Morus rubra) in the U.S.A.
نویسندگان
چکیده
منابع مشابه
Whole-Genome Characterization of Prunus necrotic ringspot virus Infecting Sweet Cherry in China
Prunus necrotic ringspot virus (PNRSV) causes yield loss in most cultivated stone fruits, including sweet cherry. Using a small RNA deep-sequencing approach combined with end-genome sequence cloning, we identified the complete genomes of all three PNRSV strands from PNRSV-infected sweet cherry trees and compared them with those of two previously reported isolates.
متن کاملExpression of Prunus Necrotic Ringspot Virus Coat Protein in E. coli
Background and Amis: Serological assay is considered as one of the best choices for conducting large number of infection tests. Recombinant DNA technology has been used for expression of virus coat protein (CP) gene in prokaryotic bacterial cells such as Escherichia coli and the recombinant CP (rCP) is used as immunogen in antibody production. Heterologous CP protein expression and purification...
متن کاملAdaptive covariation between the coat and movement proteins of prunus necrotic ringspot virus.
The relative functional and/or structural importance of different amino acid sites in a protein can be assessed by evaluating the selective constraints to which they have been subjected during the course of evolution. Here we explore such constraints at the linear and three-dimensional levels for the movement protein (MP) and coat protein (CP) encoded by RNA 3 of prunus necrotic ringspot ilarvi...
متن کاملVertical transmission of Prunus necrotic ringspot virus: hitch-hiking from gametes to seedling.
The aim of this work was to follow Prunus necrotic ringspot virus (PNRSV) infection in apricot reproductive tissues and transmission of the virus to the next generation. For this, an analysis of viral distribution in apricot reproductive organs was carried out at different developmental stages. PNRSV was detected in reproductive tissues during gametogenesis. The virus was always present in the ...
متن کاملRNA-binding properties and mapping of the RNA-binding domain from the movement protein of Prunus necrotic ringspot virus.
The movement protein (MP) of Prunus necrotic ringspot virus (PNRSV) is involved in intercellular virus transport. In this study, putative RNA-binding properties of the PNRSV MP were studied. The PNRSV MP was produced in Escherichia coli using an expression vector. Electrophoretic mobility shift assays (EMSAs) using DIG-labelled riboprobes demonstrated that PNRSV MP bound ssRNA cooperatively wit...
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ژورنال
عنوان ژورنال: Plant Disease
سال: 2020
ISSN: 0191-2917,1943-7692
DOI: 10.1094/pdis-09-19-2048-pdn