First Report of <i>Fusarium equiseti</i> Causing Seedling Death on Sugar Beet in Minnesota, U.S.A.

HomePlant DiseaseVol. 105, No. 7First Report of Fusarium equiseti Causing Seedling Death on Sugar Beet in Minnesota, U.S.A. PreviousNext DISEASE NOTE OPENOpen Access licenseFirst U.S.A.M. F. R. Khan, Y. Liu, M. Z. Bhuiyan, D. Lakshman, and S. ZhongM. Khan†Corresponding author: Khan; E-mail Address: [email protected]://orcid.org/0000-0001-5294-8651Department Plant Pathology, North Dakota State University, Fargo, ND 58102University St. Paul, MN 55108Search for more papers by this author, LiuDepartment 58102Search BhuiyanDepartment LakshmanUSDA-ARS, BARC, Beltsville, MD 20705Search Liuhttps://orcid.org/0000-0002-8106-9186Department Zhonghttps://orcid.org/0000-0003-1928-0877Department author AffiliationsAuthors Affiliations Khan1 2 † Liu1 Bhuiyan1 Lakshman3 Zhong1 1Department 58102 2University 55108 3USDA-ARS, 20705 Published Online:6 Aug 2021https://doi.org/10.1094/PDIS-10-20-2102-PDNAboutSectionsView articlePDFPDF PlusSupplemental ToolsAdd to favoritesDownload CitationsTrack Citations ShareShare onFacebookTwitterLinked InRedditEmailWechat View articleIn May 2019, sugar beet (Beta vulgaris L.) seedlings with symptoms wilting root tip discoloration necrosis were found Moorhead (46.5507° N, 96.4208° W), Roots infected surface sterilized 10% bleach 15 s, rinsed sterile distilled water, cultured water agar (MA MoorAgar, U.S.A.) 3 days at 23 ± 2°C. Isolates transferred carnation leaf (CLA) incubated room temperature (22°C) under fluorescent light 14 days. Abundant macroconidia produced sporodochia. Macroconidia five- seven-septate, slightly curved the apex, ranged from 35 110 × 1.2 3.8 µm. No microconidia produced. Chlamydospores thick, roughened walls observed chains or clumps, they ellipsoidal subglobose. A single spore was CLA potato dextrose (HiMedia Laboratories, India), abundant white mycelium, pale brown where colony contact medium. The morphological features isolates consistent (Corda) Sacc. (Leslie Summerell 2006; Li et al. 2015). Genomic DNA (Norgen Biotek, Fungi Isolation Kit no. 26200) two representative used polymerase chain reaction (PCR). second largest subunit RNA (RPB2) amplified PCR primers 5f2/7cr (O’Donnell 2010). product sequenced deposited GenBank (accession MW048778). BLAST search MLST database showed 100% sequence alignment accessions MK077037.1 NRRL 25795, respectively. Pathogenicity testing done using three (Hilleshög proprietary material, Hilleshög Seed, cotyledonary stage grown a pot (4 4 6 in.) six replicates. Seedlings inoculated conidial suspension (104 conidia/ml 8 min) root-dip method (Hanson 2006). Mock-inoculated plants dipped water. Inoculated control placed greenhouse 25 2°C 75 85% relative humidity. One week later, tissue similar those field, noninoculated symptomless. This study repeated. fungus reisolated diseased roots confirmed be based characters. reported freshly harvested stored Europe but not pathogenic (Christ 2011). Strausbaugh Gillen (2009) association rot did report pathogenicity. pathogen is several crops including edible beans that are rotation production areas (Jacobs 2018). most important species cause significant economic damage include oxysporum secorum (Secor 2014; Webb 2012). presence another will require monitoring determine how widespread it whether current commercial cultivars resistant. To our knowledge, first causing disease U.S.A.The author(s) declare no conflict interest.References:Christ, S., 2011. Phytopathology 101:1330. https://doi.org/10.1094/PHYTO-01-11-0002 Link, ISI, Google ScholarHanson, L. E. 2006. Dis. 90:1554. https://doi.org/10.1094/PD-90-1554B ScholarJacobs, J. L., 2018. 102:2035. https://doi.org/10.1094/PDIS-02-18-0332-PDN ScholarLeslie, F., Summerell, B. A., eds. Laboratory Manual. Blackwell, Ames, IA. https://doi.org/10.1002/9780470278376 Crossref, ScholarLi, G., 2015. 99:1272. https://doi.org/10.1094/PDIS-10-14-1074-PDN ScholarO’Donnell, K., 2010. Clin. Microbiol. 48:3708. https://doi.org/10.1128/JCM.00989-10 ScholarSecor, G. 2014. Fungal Biol. 118:764. https://doi.org/10.1016/j.funbio.2014.06.001 ScholarStrausbaugh, C. Gillen, A. 2009. Can. Pathol.-Revue Phytopathol. 31:232. https://doi.org/10.1080/07060660909507596 ScholarWebb, K. M., 2012. Res. 49:38. https://doi.org/10.5274/jsbr.49.1.38 ScholarThe interest.Funding: Funding provided Board Agricultural Research Education Sugarbeet Minnesota Dakota.DetailsFiguresLiterature CitedRelated Vol. 7 July 2021SubscribeISSN:0191-2917e-ISSN:1943-7692 DownloadCaptionLeaf spot Zizyphus mauritiana field caused Colletotrichum spp. (J. Shu al.). Photo credit: Q. Li. Symptoms twig dieback tea solani (A. Pandey Pandey. Metrics Downloaded 464 times Article History Issue Date: Oct 2021Published: 2021First Look: 18 Feb 2021Accepted: 10 2021 Page: 2017 Information© American Phytopathological SocietyFundingNorth EducationSugarbeet DakotaKeywordsfungivegetablespathogen detectionThe interest.