First Record of Free-Living Nematode Mylonchulus hawaiiensis from Bondowoso Regency-Indonesia

Monochids are predatory nematodes that can be found in various ecosystems. In this study, we analyzed monochids from the rhizosphere of Arabica coffee Bondowoso Regency-Indonesia. The morphological analysis showed nematode belonged to Mylonchulus hawaiiensis (Cassidy, 1931) species. results were also confirmed by a molecular using DNA single nematode. study involved polymerase chain reaction process forward primer SSU F07 (5’-AAA GAT TAA GCC ATG CAT G-3’) and reverse R 81 (5’-TGA TCC WKC YGC AGG TTC AC-3’), which amplifies small subunit (SSU) region rDNA. Phylogenetic sequence obtained formed cluster with those M. GenBank. sequences had homology ranging 99.64 99.70% compared This is first report Indonesia.