First Real-Time PCR in Morocco for Human Leptospirosis Using TaqMan Probes Targeting the LipL32 Gene
نویسندگان
چکیده
منابع مشابه
Usefulness of real-time PCR assay targeting lipL32 gene for diagnosis of human leptospirosis in Uruguay.
INTRODUCTION Assays based on DNA amplification can provide information that contributes to the initial management of patients with leptospirosis. However, these have not been adopted in Uruguay. Our aim was to evaluate the performance of the lipL32 real-time PCR (qPCR) for diagnosis of leptospirosis. METHODOLOGY We analyzed by microscopic agglutination test (MAT) and lipL32 qPCR serum samples...
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The multiplexing capabilities with different fluorescent dyes are limited in real-time PCR instruments equipped with one excitation source. Considering this limitation, a design was developed to create a triple-labeled probe as an internal positive control (IPC) that utilizes a combination of the fluorescence resonance energy transfer (FRET) and TaqMan techniques. The IPC probe, labeled with FA...
متن کاملDevelopment and validation of a modified TaqMan based real-time PCR assay targeting the lipl32 gene for detection of pathogenic Leptospira in canine urine samples.
A modified TaqMan real-time polymerase chain reaction targeting a 138bp fragment within the lipl32 gene was developed to identify exclusively pathogenic Leptospira spp. in dog urine samples. Thirty-five samples from dogs with suspected clinical leptospirosis and 116 samples from apparently healthy dogs were tested for presence of leptospiral DNA using the TaqMan-based assay. The results were co...
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Vibrio parahaemolyticus is found throughout the marine environment and is a major cause of foodborne illness around the world. Foodborne illnesses due to V. parahaemolyticus infections frequently trace back to the consumption of raw or undercooked seafood. The ability to detect V. parahaemolyticus in a rapid, sensi tive, and specific manner is important to safeguard our food supply and prevent...
متن کاملDiagnostic Accuracy of Real-Time PCR Assays Targeting 16S rRNA and lipl32 Genes for Human Leptospirosis in Thailand: A Case-Control Study
BACKGROUND Rapid PCR-based tests for the diagnosis of leptospirosis can provide information that contributes towards early patient management, but these have not been adopted in Thailand. Here, we compare the diagnostic sensitivity and specificity of two real-time PCR assays targeting rrs or lipL32 for the diagnosis of leptospirosis in northeast Thailand. METHODS/PRINCIPAL FINDINGS A case-con...
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ژورنال
عنوان ژورنال: Archives of Clinical Infectious Diseases
سال: 2019
ISSN: 2345-2641
DOI: 10.5812/archcid.67237