First Commercial Semen Cryopreservation and Main Spermatological Features of Anatolian Buffalo

Conventional buffalo semen freezing studies are limited in Anatolian buffaloes, which overly sensitive to exogenous stimulation. The present study's object was determine the main features of obtained by artificial vagina method for first time. A total number 150 ejaculates were collected from three Buffalo bulls (app. 4 years age). mean pH, volume and concentration found 6.63±0.15, 1.61±0.5 ml, 1629±222.67 x106 spermatozoa/ml, respectively. sperm motion characteristics determined using a computer-assisted analysis system (CASA); progressively motile values 57.12±5.63%, 23.22±4.47% other kinetic parameters such VAP, VSL, VCL, ALH, BCF, STR, LIN 94.71±8.48 µm/s, 72.6±7.08 160.9±15.66 7.8±3.75 µm, 29.15±1.56 Hz, 76.91±3.87%, 46.21±2.61%, respectively after thawing. Among bulls, differences pH statistically significant (P>0.05), while ejaculate volume, concentration, motility, progressive not (P>0.05). As result, frozen can be economically used animal breeding assisted reproductive biotechnology as insemination or vitro embryo production commercially.