Filtration leukapheresis (FL) and granulocytes [letter]

Filtration leukapheresis (FL) and granulocytes.

Damage of granulocytes in filtration leukapheresis (FL).

To The Editor: To elucidate the mechanism of granulocyte damage during FL, studies have been reported of the interaction between nylon-wool fibers and isolated granulocytes suspended in protein free media,’2 in 5% plasma,2 or in 1% human serum albumin.3 In FL, however, heparinized whole blood is passed through a nylon-wool filter, resulting in plasma protein coating of the fibers within seconds...

In vitro function and post-transfusion survival of granulocytes collected by continuous-flow centrifugation and by filtration leukapheresis.

The function of granulocytes collected by continuous-flow centrifugation (CFC) and by filtration leukapheresis (FL) was studied in vitro, and the post-transfusion recovery and intravascular survival of these cells was studied by autologous transfusion in normal donors. Granulocytes collected by both FL and CFC leukapheresis (CFCL) functioned normally in the quantitative nitroblue tetrazolium, o...

Mobilization and exocytosis of specific (secondary) granules by human neutrophils during adherence to nylon wood in filtration leukapheresis (FL).

We studied the interaction of human neutrophils with nylon wool in vitro and during filtration Ieukapheresis (FL) to better understand cellular events detrimental to neutrophils that occur during FL. When neutrophils purified from blood by gradient centrifugation and sedimentation were incubated with nylon wool in vitro, release of lysozyme from the cells occurred rapidly and to a degree that w...

Pretreatment of filtration leukapheresis donors with colchicine.

Although filtration leukapheresis (FL) is a relatively simple and efficient means of harvesting neutrophils (PMN) for transfusion, the PMN obtained by this procedure have both functional and morphologic abnormalities induced when the cells adhere to nylon wool filters used in FL. Adherence to nylon wool causes a partial degranulation of PMN together with the production of superoxide at cell sur...