Filling a GAP—An Optimized Probe for ER Ca 2+ Imaging In Vivo
نویسندگان
چکیده
منابع مشابه
GFP-Aequorin Protein Sensor for Ex Vivo and In Vivo Imaging of Ca(2+) Dynamics in High-Ca(2+) Organelles.
Proper functioning of organelles such as the ER or the Golgi apparatus requires luminal accumulation of Ca(2+) at high concentrations. Here we describe a ratiometric low-affinity Ca(2+) sensor of the GFP-aequorin protein (GAP) family optimized for measurements in high-Ca(2+) concentration environments. Transgenic animals expressing the ER-targeted sensor allowed monitoring of Ca(2+) signals ins...
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ژورنال
عنوان ژورنال: Cell Chemical Biology
سال: 2016
ISSN: 2451-9456
DOI: 10.1016/j.chembiol.2016.06.003