Fifty Seychelles warbler (Acrocephalus sechellensis) microsatellite loci polymorphic in Sylviidae species and their cross-species amplification in other passerine birds
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Polymorphic microsatellite loci from the American goldfinch ( Carduelis tristis ) and their cross-amplification in a variety of passerine species
Cardueline finches have become important models in studies of sexual selection. Here I describe eight polymorphic microsatellite loci isolated from the American goldfinch ( Carduelis tristis ) that are useful in parentage and heterozygosity studies. The number of alleles per locus ranged from eight to 38 based on 252 goldfinches. The markers are polymorphic in at least four other Cardueline fin...
متن کاملMHC diversity in two Acrocephalus species: the outbred Great reed warbler and the inbred Seychelles warbler.
The Great reed warbler (GRW) and the Seychelles warbler (SW) are congeners with markedly different demographic histories. The GRW is a normal outbred bird species while the SW population remains isolated and inbred after undergoing a severe population bottleneck. We examined variation at Major Histocompatibility Complex (MHC) class I exon 3 using restriction fragment length polymorphism, denatu...
متن کاملParentage assignment and extra-group paternity in a cooperative breeder: the Seychelles warbler (Acrocephalus sechellensis).
We describe the development and initial application of a semiautomated parentage testing system in the Seychelles warbler (Acrocephalus sechellensis). This system used fluorescently labelled primers for 14 polymorphic microsatellite loci in two multiplex loading groups to genotype efficiently over 96% of the warbler population on Cousin island. When used in conjunction with the program CERVUS, ...
متن کاملPolymorphic microsatellite loci for the genetic analysis of Lycoris radiata (Amaryllidaceae) and cross-amplification in other congeneric species.
Lycoris radiata is a perennial herb that has been used in traditional Chinese medicine for a long time and has two main medicinal components in its bulb, lycorine and galanthamine. However, the original microsatellite loci have not been developed for any species of Lycoris. Total genomic DNA was extracted from fresh bulbs using a modified CTAB protocol. We isolated 10 microsatellite loci ...
متن کاملNew microsatellite loci for Narcissus papyraceus (Amarillydaceae) and cross-amplification in other congeneric species.
UNLABELLED PREMISE OF THE STUDY Microsatellite loci from a genomic library of the species Narcissus papyraceus were optimized and characterized for studies of population genetics. • METHODS AND RESULTS Eleven markers that were successfully amplified showed polymorphism when tested on 50 individuals from two populations in southern Spain and northern Morocco. Overall, the number of alleles ...
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ژورنال
عنوان ژورنال: Molecular Ecology
سال: 2000
ISSN: 0962-1083,1365-294X
DOI: 10.1046/j.1365-294x.2000.105338.x