منابع مشابه
Culture of fetal cells from maternal blood for prenatal diagnosis.
The isolation and analysis of fetal cells from maternal blood would allow non-invasive prenatal genetic screening and diagnosis. Over the past decade, progress has been made towards this goal using various enrichment strategies and analysis by fluorescence in-situ hybridization with chromosome-specific probes and PCR. One method that is currently being explored involves culturing fetal cells. D...
متن کاملNoninvasive diagnosis of fetal aneuploidy by shotgun sequencing DNA from maternal blood.
We directly sequenced cell-free DNA with high-throughput shotgun sequencing technology from plasma of pregnant women, obtaining, on average, 5 million sequence tags per patient sample. This enabled us to measure the over- and underrepresentation of chromosomes from an aneuploid fetus. The sequencing approach is polymorphism-independent and therefore universally applicable for the noninvasive de...
متن کاملNon-invasive prenatal diagnosis of fetal trisomy 21 using cell-free fetal DNA in maternal blood
Since the existence of cell-free fetal DNA (cff-DNA) in maternal circulation was discovered, it has been identified as a promising source of fetal genetic material in the development of reliable methods for non-invasive prenatal diagnosis (NIPD) of fetal trisomy 21 (T21). Currently, a prenatal diagnosis of fetal T21 is achieved through invasive techniques, such as chorionic villus sampling or a...
متن کاملIsolation of fetal nucleated red blood cell from maternal blood using immunomagnetic beads for prenatal diagnosis.
The immunomagnetic beads method for isolation of fetal nucleated red blood cells (FNRBCs) from peripheral blood of 78 pregnant women for prenatal diagnosis was developed. The study subjects were classified into 8-10 and 11-14 weeks of gestation (n = 39 each). Peripheral blood cells were divided into two for the FNRBCs isolation using two protocols, one with anti-CD45 depletion followed by anti-...
متن کامل[Prenatal diagnosis by the presence of fetal DNA in maternal blood].
El meticuloso e interesante artículo de Sesarini y colaboradores, publicado en este número de Archivos1, provee por primera vez datos locales sobre el aislamiento de ADN fetal circulante en forma libre en sangre materna, y su análisis para determinar grupo RhD y sexo del feto. La presencia de ADN fetal en plasma y suero materno fue demostrada por vez primera en 19972 y confirmada luego en numer...
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ژورنال
عنوان ژورنال: Japanese Journal of Thrombosis and Hemostasis
سال: 1999
ISSN: 1880-8808,0915-7441
DOI: 10.2491/jjsth.10.174