Fertilization Rate of Giant Grouper (Epinephelus lanceolatus, Bloch 1790) Sperm Post-cryopreservation and Application on Hybridization in Different Grouper Species

Abstract Fertilization was doing to measure the success of sperm cryopreservation because it more informative. Sperm expected get that successfully fertilize egg well. The aim this study determine fertilization ability giant grouper (Epinephelus lanceolatus, Bloch 1790) 48 hours post-cryopreservation in fertilizing tiger eggs fuscoguttatus, Forskal 1775). carried out artificially using with 6% glycerol and palm dates concentrates (0%; 5%; 10%; 15%; 20%; 25%). brood previously injected hormone chorionic gonadotropin at a dose 500 IU/kg. results showed there were significant differences (P < 0.05) on which cryopreserved various concentrations date concentrates. 10% concentrate has best fertility by showing highest value 66.25 ± 3.23%.