Fermentation of Cellulosic Substrates in Batch and Continuous Culture by Clostridium thermocellum
نویسندگان
چکیده
منابع مشابه
Continuous cellulosic bioethanol fermentation by cyclic fed-batch cocultivation.
Cocultivation of cellulolytic and saccharolytic microbial populations is a promising strategy to improve bioethanol production from the fermentation of recalcitrant cellulosic materials. Earlier studies have demonstrated the effectiveness of cocultivation in enhancing ethanolic fermentation of cellulose in batch fermentation. To further enhance process efficiency, a semicontinuous cyclic fed-ba...
متن کاملCellulose fermentation by Clostridium thermocellum and a mixed consortium in an automated repetitive batch reactor.
An automated repetitive batch fermentation system was developed to facilitate the study of microbial cellulose utilization. The system was operated with Avicel as the carbon source and either Clostridium thermocellum ATCC 27405 or a consortium enriched from compost as inocula. Multiple cycles of growth medium addition, incubation, and medium removal were performed with each inoculum. Removal an...
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Clostridium thermocellum is a thermophilic, anaerobic, cellulolytic bacterium that produces ethanol and acetic acid as major fermentation end products. The effect of growth conditions on gene expression in C. thermocellum ATCC 27405 was studied using cells grown in continuous culture under cellobiose or cellulose limitation over a approximately 10-fold range of dilution rates (0.013 to 0.16 h(-...
متن کاملRegulation of cellulase synthesis in batch and continuous cultures of Clostridium thermocellum.
Regulation of cell-specific cellulase synthesis (expressed in milligrams of cellulase per gram [dry weight] of cells) by Clostridium thermocellum was investigated using an enzyme-linked immunosorbent assay protocol based on antibody raised against a peptide sequence from the scaffoldin protein of the cellulosome (Zhang and Lynd, Anal. Chem. 75:219-227, 2003). The cellulase synthesis in Avicel-g...
متن کاملKinetic modeling of batch fermentation for Populus hydrolysate tolerant mutant and wild type strains of Clostridium thermocellum.
The extent of inhibition of two strains of Clostridium thermocellum by a Populus hydrolysate was investigated. A Monod-based model of wild type (WT) and Populus hydrolysate tolerant mutant (PM) strains of the cellulolytic bacterium C. thermocellum was developed to quantify growth kinetics in standard media and the extent of inhibition to a Populus hydrolysate. The PM was characterized by a high...
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ژورنال
عنوان ژورنال: Applied and Environmental Microbiology
سال: 1989
ISSN: 0099-2240,1098-5336
DOI: 10.1128/aem.55.12.3131-3139.1989