Feather buds exert a polarizing activity when transplanted to chick limb buds

Effect of Thyroxine on Early Development of Chick Limb Buds

Congenic method in the chick limb buds by electroporation.

Electroporation is a powerful tool with which to study limb development. Limb development, however, remains an intricate series of events, requiring the precise dissection of developmental processes using relevant transgenes. In this review, we describe the anatomy of the limb field as the basis of targeted electroporation, and specific expression vectors are discussed. We share a useful protoc...

Building limb buds

This workshop on limb development was a highly enjoyable meeting that covered a wide range of topics related to appendage development and evolution. The discussions were vivid, open and stimulating for all participants. It was particularly interesting to see how broadly accepted concepts, such as the morphogen models in the fly and the progress zone model in vertebrates, were re-evaluated on th...

Gradients of homeoproteins in developing feather buds.

Homeoproteins are functionally involved in pattern formation. Recently, homeoproteins have been shown to be distributed in a graded fashion in developing limb buds. Here we examine the expression of homeoproteins in chicken feather development by immunocytochemical localization. We find that XlHbox 1 antigen is present in cell nuclei and is distributed in a gradient in the mesoderm of developin...

Replication-defective virus infection of feather buds produces a localized region of beta-galactosidase activity.

We are interested in using retroviral vectors to trace cell lineage and to introduce exogenous genes in chicken skin explant cultures. Here the LZ10 virus carrying the gene encoding beta-galactosidase was introduced to the skin explants by two different means: a) the virus was added to the media or b) the virus was microinjected into regions of the developing feather buds. Infection by microinj...