Fast kinetic studies with chiroptical techniques: Stopped flow circular dichroism and related methods

Improved stability of the Jun-Fos Activator Protein-1 coiled coil motif: A stopped-flow circular dichroism kinetic analysis.

Two c-Jun leucine zipper variants that bind with high affinity to c-Fos have been selected using semirational design combined with protein-fragment complementation assays (JunW) or phage display selection (JunW(Ph1)). Enriched winners differ from each other in only two of ten semi-randomized positions, with DeltaT(m) values of 28 degrees C and 37 degrees C over wild-type. cFos-JunW, cFos-JunW(P...

Single Molecule Chiroptical Spectroscopy: Fluorescence Excitation Circular Dichroism and Circular Polarized Luminescence of Bridged Triarylamine Helicenes

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Biomolecular studies by circular dichroism.

In this review, we shall outline the basic principles of circular dichroism (CD) indicating the types of structural information relevant to the study of biomolecules, such as proteins or DNA. We are mainly interested to show the utility of this technique to study protein-ligand, DNA-ligand and protein-DNA interactions.

A stopped-flow/flow-injection system for automation of α2 macroglobulin kinetic studies

Binding of a calcium sensitizer, bepridil, to cardiac troponin C. A fluorescence stopped-flow kinetic, circular dichroism, and proton nuclear magnetic resonance study.

Stopped-flow fluorescence kinetic measurements, circular dichroism (CD), and 1H nuclear magnetic resonance (NMR) spectroscopy at 360 MHz have been used to study the interaction of the calcium-channel blocker and calmodulin antagonist bepridil with cardiac troponin C (cTnC) in the presence of calcium. The kinetic data show that bepridil reduces the rate of calcium release only from the low affin...