Fast high performance liquid chromatography separations for proteomic applications using Fused-Core? silica particles

Phenylene-bridged hybrid silica spheres for high performance liquid chromatography†

Highly monodisperse 1,3-phenylene-bridged hybrid organosilica spheres (m-PHS) were synthesized by co-condensation of tetraethoxysilane (TEOS) and 1,3-bis(triethoxysilyl)benzene (1,3-BTEB) using dodecylamine (DDA) and cetyltrimethylammonium bromide (CTAB) as templates. In this method, three important factors are the surfactants, the ethanol–water volume ratio and the TEOS/1,3-BTEB molar ratio. T...

Fused-core particle technology in high-performance liquid chromatography: An overview

The advent of superficially porous particles (SPPs) for packed HPLC columns has changed the way that many practitioners have approached the problem of developing needed separations. The very high efficiency of such columns, combined with convenient operating conditions, modest back pressures and the ability to use conventional HPLC instruments has resulted in intense basic studies of SPP techno...

Chiral Separations: Liquid Chromatography

The use of CE in the analysis of pharmaceuticals is now becoming Rrmly established as a useful complement and alternative to the more widely employed technique of HPLC. The major attractions of CE are that considerable cost and time savings are possible, especially in the areas of chiral analysis and in the determination of solutes having limited or no chromophore. The recent advent of the use ...

Core-Shell, Ultrasmall Particles, Monoliths, and Other Support Materials in High-Performance Liquid Chromatography.

Optimized high performance liquid chromatography–ultraviolet detection method using core-shell particles for the therapeutic monitoring of methotrexate☆

Methotrexate (MTX) is an antineoplastic drug, and due to its high toxicity, the therapeutic drug monitoring is strictly conducted in the clinical practice. The chemometric optimization and validation of a high performance liquid chromatography (HPLC) method using core-shell particles is presented for the determination of MTX in plasma during therapeutic monitoring. Experimental design and respo...