منابع مشابه
Northern blots for small RNAs and microRNAs.
This protocol describes the detection of small RNAs (~10-200 nucleotides) by blot hybridization. The RNA samples, denatured in formamide, are separated by denaturing polyacrylamide gel electrophoresis. Because high-percentage polyacrylamide gels are required to separate RNAs in this size range, it is necessary to perform electrophoretic transfer to positively charged nylon membranes. After tran...
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Northern blots enable detection of a target RNA of interest in a biological sample using standard benchtop equipment. miRNAs are the most challenging targets as they must be detected with a single short nucleic acid probe. With existing approaches, it is cumbersome to perform multiplexed blots in which several RNAs are detected simultaneously, impeding the study of interacting regulatory elemen...
متن کاملDirect cloning of differential display products eluted from northern blots.
To identify novel or previously described mRNAs that are differentially expressed, numerous investigators have used differential display (DD) (3,4). Briefly, DD involves the reverse-transcription (RT) of mRNA to singlestranded cDNA followed by polymerase chain reaction (PCR) amplification. The PCR conditions and primers used generate a sub-population of cDNA fragments corresponding to the 3′ en...
متن کاملFast and Simple Scatterplot
An important element of both exploratory data analysis and many dimensionality reduction techniques is a scatterplot smoother. In both areas there is a strong need for fast and simple procedures. A major hurdle is choice of the amount of smoothing. In this paper we propose a fast and simple choice of the scatterplot smoothing parameter, based on blockwise least squares parabolic tting. Our meth...
متن کاملSimple and Fast Inverse Alignment
For as long as biologists have been computing alignments of sequences, the question of what values to use for scoring substitutions and gaps has persisted. While some choices for substitution scores are now common, largely due to convention, there is no standard for choosing gap penalties. An objective way to resolve this question is to learn the appropriate values by solving the Inverse String...
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ژورنال
عنوان ژورنال: Biochemistry Insights
سال: 2009
ISSN: 1178-6264,1178-6264
DOI: 10.4137/bci.s2257