Factors influencing fractionation of DNA restriction fragments by RPC-5 column chromatography.

Preparative fractionation of DNA restriction fragments by reversed phase column chromatography.

Reversed phase column chromatography on RPC-5 resin was used to fractionate milligram quantities of DNA fragments generated by restriction endonucleases. Fractionation was on the basis of size, the presence or absence of sticky ends, and at least one as yet undertermined property.

chromatography on RPC - 5 1 1 2 3 2

High pressure liquid chromatography on the RPC-5 reversed-phase ion exchange system has been shown to have several potential applications as an initial high capacity step in the isolation of specific DNA restriction fragments. The fractionation of a Hinc II digest of X DNA, which contains 35 fragments with "flush ends" ranging in size from 3 x 10 to 7 x 10 daltons, has been used as a model syst...

Microfluidic Devices for Fractionation of Dna Fragments

The fraction collection of DNA fragments for studying gene expression, suffered from technical difficulties, including those related to the accurate capture of fast moving targets and pure collection under conditions of insufficient separation. We introduces a new concept of simultaneous space sampling for capturing targets in complicated situation, like overlapping fragments. Ten parallel extr...

Fractionation of heparin by chromatography on a tissue plasminogen activator-Sepharose column.

Heparin stimulates the activity of tissue plasminogen activator (t-PA) and binds to t-PA. To study this interaction, a complex between t-PA and N-acetylated heparin was formed and then linked to Sepharose. This procedure selectively links the t-PA to the column because the acetylated heparin has no free amino groups. The procedure also protects the heparin-binding site(s) on the enzyme during c...

Fractionation of PCDDs, PCBs, and pesticides by column chromatography on exfoliated graphites.