Factors Influencing Alpha-toxin Production by Clostridium perfringens
نویسندگان
چکیده
منابع مشابه
Nutritional Requirements of Clostridium Perfringens Pb6k for Alpha Toxin Production
A potent toxin was produced by a strain of Clostridium perfringens PB6K in a chemically defined medium consisting of 19 amino acids, fructose, vitamins and minerals. A large amount of L-arginine (10 mg/ml or higher concentration) was required for toxin production. An excess of L-cystine (0.1 mg/ml or higher) reduced the yield of toxin seriously. Zinc and manganese, though ineffective for growth...
متن کاملRegulation of Toxin Production in Clostridium perfringens
The Gram-positive anaerobic bacterium Clostridium perfringens is widely distributed in nature, especially in soil and the gastrointestinal tracts of humans and animals. C. perfringens causes gas gangrene and food poisoning, and it produces extracellular enzymes and toxins that are thought to act synergistically and contribute to its pathogenesis. A complicated regulatory network of toxin genes ...
متن کاملMedium for toxin production by Clostridium perfringens in continuous culture.
A tryptone-salts medium for continuous growth and alpha toxin production of Clostridium perfringens type A in an adapted chemostat is described. In such steady-state cultures, fermentative and biochemical activity of C. perfringens remained unchanged. Toxigenic ability to produce alpha, theta, and nu toxins was preserved.
متن کاملFactors Involved in Production of Clostridium Welchii Alpha Toxin
Maximum production of the alpha toxin by Cl. welchii is dependent on the inclusion in the medium of several substances in addition to those required for growth. These factors include: 1. Some substance present in enzymatic digests of certain proteins such as casein and gelatine. 2. Glycerylphosphorylcholine and other substances that are present in extracts of pancreas. 3. The use of starch or d...
متن کاملMembrane-Binding Mechanism of Clostridium perfringens Alpha-Toxin
Clostridium perfringens alpha-toxin is a key mediator of gas gangrene, which is a life-threatening infection that manifests as fever, pain, edema, myonecrosis, and gas production. Alpha-toxin possesses phospholipase C and sphingomyelinase activities. The toxin is composed of an N-terminal domain (1-250 aa, N-domain), which is the catalytic site, and a C-terminal domain (251-370 aa, C-domain), w...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Nippon Saikingaku Zasshi
سال: 1963
ISSN: 1882-4110,0021-4930
DOI: 10.3412/jsb.18.344