Factors in Tissue Handling and Processing That Impact RNA Obtained From Formalin-fixed, Paraffin-embedded Tissue
نویسندگان
چکیده
منابع مشابه
Proteomic Sample Preparation from Formalin Fixed and Paraffin Embedded Tissue
Preserved clinical material is a unique source for proteomic investigation of human disorders. Here we describe an optimized protocol allowing large scale quantitative analysis of formalin fixed and paraffin embedded (FFPE) tissue. The procedure comprises four distinct steps. The first one is the preparation of sections from the FFPE material and microdissection of cells of interest. In the sec...
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Several DNA extraction methods have been used for formalin-fixed, paraffin-embedded tissues, with variable results being reported regarding the suitability of DNA obtained from such sources to serve as template in polymerase chain reaction (PCR)-based genetic analyses. We present a method routinely used for archival material in our laboratory that reliably yields DNA of sufficient quality for P...
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Title of dissertation: PROTEOME ANALYSIS OF FORMALIN-FIXED AND PARAFFINEMBEDDED TISSUE Tong Guo, Doctor of Philosophy, 2008 Dissertation directed by: Professor Cheng S. Lee Department of Chemistry and Biochemistry Because of the long history of the use of formalin as the standard fixative for tissue processing in histopathology, these archival formalin-fixed and paraffinembedded (FFPE) tissues ...
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Biofluid detectable biomarkers that originate at the site of diseased tissues would be advantageous, in that, they may provide mechanistic information concerning the manifestation and progression of the disease. Unfortunately, tissue biopsies are precious samples that can generally be acquired in small amounts due to the invasive nature of the sample collection. One of the foundations of pathol...
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The present study aimed to compare two different methods of extracting RNA from formalin-fixed paraffin-embedded (FFPE) specimens of diffuse large B-cell lymphoma (DLBCL). We further aimed to identify possible influences of variables--such as tissue size, duration of paraffin block storage, fixative type, primers used for cDNA synthesis, and endogenous genes tested--on the success of amplificat...
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ژورنال
عنوان ژورنال: Journal of Histochemistry & Cytochemistry
سال: 2008
ISSN: 0022-1554,1551-5044
DOI: 10.1369/jhc.2008.951863