Factors affecting growth and nitrogen fixation of Spirillum lipoferum

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The nitrogenase system of Spirillum lipoferum.

The nitrogenase system of Spirillum lipoferum was separated into three components, the normal Mo-Fe and Fe proteins as well as an activating factor for the Fe protein. The rate of activation is increased by Mn2+ or by an excess of Mg2+, and the process requires ATP.

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Interaction of Azospirillum lipoferum with wheat germ agglutinin stimulates nitrogen fixation.

In vitro, the nitrogen fixation capability of A. lipoferum is efficiently increased in the presence of wheat germ agglutinin (WGA). A putative WGA-binding receptor, a 32-kDa protein, was detected in the cell capsule. The stimulatory effect required N-acetyl-D-glucosamine dimer (GlcNAcdi) terminated sugar side chains of the receptor and was dependent on the number of GlcNAcdi links involved in r...

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ژورنال

عنوان ژورنال: Journal of Bacteriology

سال: 1976

ISSN: 0021-9193,1098-5530

DOI: 10.1128/jb.127.3.1248-1254.1976