Factorial and time course designs for cDNA microarray experiments
نویسندگان
چکیده
منابع مشابه
Factorial and time course designs for cDNA microarray experiments.
Microarrays are powerful tools for surveying the expression levels of many thousands of genes simultaneously. They belong to the new genomics technologies which have important applications in the biological, agricultural and pharmaceutical sciences. There are myriad sources of uncertainty in microarray experiments, and rigorous experimental design is essential for fully realizing the potential ...
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We consider cDNA microarray experiments when the cell populations have a factorial structure, and investigate the problem of their optimal designing under a baseline parametrization where the objects of interest differ from those under the more common orthogonal parametrization. First, analytical results are given for the 2× 2 factorial. Since practical applications often involve a more complex...
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We calculate the variances of two classes of estimates of differential gene expression based on log ratios of fluorescence intensities from cDNA microarray experiments: direct estimates, using measurements from the same slide, and indirect estimates, using measurements from different slides. These variances are compared and numerical estimates are obtained from a small experiment involving 4 sl...
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In this paper, optimal incomplete block designs were determined for cDNA microarray experiments, where arrays are blocks, block size is always two, and the number of blocks or arrays available is approximately equal to the number of samples. A new class of designs, the mixed designs, which are a mixture of reference and loop designs, have been identified and their optimality has been proven in ...
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Characterizing the genome-wide dynamic regulation of gene expression is important and will be of much interest in the future. However, there is currently no established method for identifying differentially expressed genes in a time course study. Here we propose a significance method for analyzing time course microarray studies that can be applied to the typical types of comparisons and samplin...
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ژورنال
عنوان ژورنال: Biostatistics
سال: 2004
ISSN: 1465-4644,1468-4357
DOI: 10.1093/biostatistics/5.1.89