Facile preparation and characterization of the toxin from Bacillus thuringiensis var. kurstaki
نویسندگان
چکیده
منابع مشابه
Facile autoplast generation and transformation in Bacillus thuringiensis subsp. kurstaki.
We describe a method for maximizing the rate of conversion of Bacillus thuringiensis subsp. kurstaki vegetative cells to osmotically fragile forms in the absence of exogenously added enzymes. Optimal generation of autoplasts occurred in 50 mM sodium acetate buffer (pH 7.0) at 37 degrees C with 10% (wt/vol) polyethylene glycol as an osmotic stabilizer. The maximum autolytic rate resulted in a co...
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The intentional and controlled release of an aerosolized bacterium provides an opportunity to investigate the implications of a biological attack. Since 2006, Los Alamos National Laboratory has worked with several urban areas, including Fairfax County, VA, to design experiments to evaluate biodefense concepts of operations using routine spraying of Bacillus thuringiensis var. kurstaki (Btk). Bt...
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AIMS Some Cry proteins produced by the soil bacterium Bacillus thuringiensis (Bt) or by transgenic Bt plants persist in agricultural soils for an extended period of time, which may pose a hazard for nontarget soil organisms. The aims of our study were to screen for soil fungi capable of degrading the Cry1Ac toxin and to identify the mechanisms that lead to the inactivation of this protein. ME...
متن کاملSafety of Bacillus thuringiensis var. kurstaki Applications for Insect Control to Humans and Large Mammals
This minireview discusses the risks to humans and large mammals associated with the use of Bacillus thuringiensis var. kurstaki (Btk) in forest, agricultural and urban environments. The first subspecies used for insect control was Bacillus thuringiensis subsp. thuringiensis (Btt), known at the time as Bt Berliner. Much of the early work done with Bt does not identify the subspecies. Btk is regi...
متن کاملCharacterization and partial purification of a plasma membrane receptor for Bacillus thuringiensis var. kurstaki lepidopteran-specific delta-endotoxin.
The lepidopteran-specific P1 delta-endotoxin of Bacillus thuringiensis var. kurstaki HD-1 was activated in vitro using insect gut proteases and found to be highly specific for the lepidopteran cell line Choristoneura fumiferana CF1 among a wide range of lepidopteran and dipteran cell lines tested. The toxicity of P1 against CF1 cells is inhibited by N-acetylgalactosamine (GalNAc), and the lecti...
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ژورنال
عنوان ژورنال: Biochemical Journal
سال: 1989
ISSN: 0264-6021,1470-8728
DOI: 10.1042/bj2600087