Fabrication of a 3D microfluidic cell culture device for bone marrow-on-a-chip

Hot embossing for fabrication of a microfluidic 3D cell culture

Clinically relevant studies of cell function in vitro require a physiologically-representative microenvironment possessing aspects such as a 3D extracellular matrix (ECM) and controlled biochemical and biophysical parameters. A polydimethylsiloxane (PDMS) microfluidic system with a 3D collagen gel has previously served for analysis of factors inducing different responses of cells in a 3D microe...

Fabrication of Biomimetic 3d Liver Tissue Using Photodegradable Hydrogels and Perfusion Culture in a Microfluidic Device

This paper reports a novel technique to fabricate perfusable 3D liver tissue in a microfluidic device using photodegradable hydrogels prepared with gelatin. Also, we performed the perfusion culture of human hepatocellular carcinoma (HepG2) cells encapsulated in the patterned hydrogels.

Design and Fabrication of a Membrane Integrated Microfluidic Cell Culture Device Suitable for High-Resolution Imaging

Integrated microfluidic cell culture and lysis on a chip.

We present an integrated microfluidic cell culture and lysis platform for automated cell analysis that improves on systems which require multiple reagents and manual procedures. Through the combination of previous technologies developed in our lab (namely, on-chip cell culture and electrochemical cell lysis) we have designed, fabricated, and characterized an integrated microfluidic platform cap...

Hot embossing for fabrication of a microfluidic 3D cell culture platform.

Clinically relevant studies of cell function in vitro require a physiologically-representative microenvironment possessing aspects such as a 3D extracellular matrix (ECM) and controlled biochemical and biophysical parameters. A polydimethylsiloxane (PDMS) microfluidic system with a 3D collagen gel has previously served for analysis of factors inducing different responses of cells in a 3D microe...