Extradiol cleavage of 3-substituted catechols by an intradiol dioxygenase, pyrocatechase, from a Pseudomonad.
نویسندگان
چکیده
منابع مشابه
Distal cleavage of 3-chlorocatechol by an extradiol dioxygenase to 3-chloro-2-hydroxymuconic semialdehyde.
A 2,3-dihydroxybiphenyl 1,2-dioxygenase from the naphthalenesulfonate-degrading bacterium Sphingomonas sp. strain BN6 oxidized 3-chlorocatechol to a yellow product with a strongly pH-dependent absorption maximum at 378 nm. A titration curve suggested (de)protonation of an ionizable group with a pKa of 4.4. The product was isolated, purified, and converted, by treatment with diazomethane, to a d...
متن کاملExtradiol cleavage of 3-methylcatechol by catechol 1,2-dioxygenase from various microorganisms.
The isofunctional enzymes of catechol 1,2-dioxygenase from species of Acinetobacter, Pseudomonas, Nocardia, Alcaligenes, and Corynebacterium oxidize 3-methylcatechol according to both the intradiol and extradiol cleavage patterns. However, the enzyme preparations from Brevibacterium and Arthrobacter have only the intradiol cleavage activity. Comparison of substrate specificity among these isofu...
متن کاملIntradiol Aromatic Ring Cleavage in a Dioxygenase Model System
Dennis G. Brown* and William J. Hughes Department of Chemistry, University of Idaho, Moscow, Idaho, USA 83843 Z. Naturforsch. 36b, 1324-1330 (1981); received March 23, 1981 Dioxygenase Model, Copper, Catechol The complex (3-n-nonylcatecholato)(l,10-phenanthroline)copper(II) reacts with molecular oxygen to give an isomeric mixture of 2-n-nonylmuconic acid and several secondary oxidation products...
متن کاملThree Isozymes of Catechol l , Z - Dioxygenase ( Pyrocatechase ) , cycy , cr / 3 , and & 3 , from
Three isozymes of catechol 1,2-dioxygenase (pyrocatechase) from Pseudomonas at-villa C-l were separated using DEAE-Toyopearl chromatography. The specific activities of each isozyme were similar to one another. The molecular weights of isozymes 1, 2, and 3 were estimated to be approximately 67,000,64,000, and 59,000, respectively, from gel filtration. On sodium dodecyl sulfate-polyacrylamide gel...
متن کاملA process optimization for bio-catalytic production of substituted catechols (3-nitrocatechol and 3-methylcatechol
BACKGROUND Substituted catechols are important precursors for large-scale synthesis of pharmaceuticals and other industrial products. Most of the reported chemical synthesis methods are expensive and insufficient at industrial level. However, biological processes for production of substituted catechols could be highly selective and suitable for industrial purposes. RESULTS We have optimized a...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1975
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(19)41246-5