Extraction, Separation and Purification of Bioactive Anticancer Components from Peganum harmala against Six Cancer Cell Lines Using Spectroscopic Techniques

Conventional cancer treatments normally involve chemotherapy or a combination of radio- and chemotherapy. However, the adverse effects synthetic medicines encouraged exploration novel therapeutic medications bio-friendly nature. In an effort to explore anticancer compounds from natural resources, crude extract Peganum harmala (seeds) was fractionated on basis polarity, fractions were further tested for activity. Brine shrimp lethality assays potato disc antitumor used test each fraction cytotoxic potential. The ethyl acetate found be most potent, with LC50 IC50 values 34.25 µg/mL 38.58 µg/mL, respectively. Further activity-guided fractionation led isolation bioactive compound PH-HM-10 which identified characterized by Mass Spectroscopy (MS), Infrared (IR), Proton Nuclear Magnetic Resonance (1HNMR), Carbon (13CNMR) Heteronuclear Single Quantum Correlation (HSQC). Anticancer aspects in isolated determined against six human cell lines maximum effect (IC50 = 36.99 µg/mL) myeloid leukemia (HL-60) line, followed lung adenocarcinoma epithelial line (A549) breast (MCF-7) 63.5 85.9 respectively). findings current study suggest that (Pegaharmine E) is significantly active can investigated develop future chemotherapeutic agents.