Extraction of Phenolic and Flavonoid from Seeds of Black Soybean (Glycine max L.)

Aims: The study was conducted on black soybean seeds (Glycine max. L) to optima the conditions of some factors affecting extracted yield polyphenol compounds.
 Study Design: include types solvent used (methanol, ethanol and acetone); concentrations (40, 50, 60, 70, 80 90 % v/v); ratio sample (1:4,1:6, 1:8, 1:10) cycle extractions (2, 3, 4); extract times 4h) temperature (30, 40, 60 oC). efficiency shown by content total phenolic (TPC) flavonoid (TFC).
 Place Duration Study: Department Biotechnology Food technology, Thai Nguyen University Agriculture Forestry, Viet Nam, from March 2021 2022.
 Methodology: were pulverized a mill defatted using Soxhlet system with hexane for 10 hrs as described Weidner et al. (2012). TPC determined Folin-Ciocalteu method Susu Giang al (2013). TFC colorimetric Ozsoy (2008).
 Results: results showed that extraction high when 70% acetone solvent; appropriate 1:10 3 cycles extraction. can be improved extracting samples at 60oC 4 hours, reached about 5.02±0.04 mgGAE/g 2.74±0.03 mgQE/g.
 Conclusion: is most suitable polyphenols soybean. in (w/v), three hours each 60°C highest TFC, reaching 7.02 ±0.04 mgQE/g, respectively.