Extraction of laminarin from Saccharina latissima seaweed using cross-flow filtration

Abstract Laminarin is a low-molecular-weight polysaccharide found in seaweed (kelp), often equal concentrations to that the commercially important hydrocolloid alginate. However, while alginate can be easily recovered by dissolution followed acid precipitation, for laminarin, there no such straightforward way of recovering it. used as dietary fiber and, if efficiently extracted, it may functional food/feed applications and component plant defense stimulants agriculture. One concentrating laminarin from dilute solutions press solution through ultrafine membranes molecules cannot pass through. When an pretreatment step residue process also contains laminarin. We cross-flow filtration concentrate Saccharina latissima , retrieving extraction. Three ceramic with 5, 15, 50 kDa molecular weight cutoffs were used, pressure, temperature, feed velocity altered reveal which parameters controlled flow membrane how was concentrated. The effects on extraction fresh vs. frozen biomass evaluated showing releases more similar homogenization technique. Thermal microbial degradation components studied during course filtrations, affect concentration, temperature ~ 65 °C had little impact techniques monitor permeate nuclear magnetic resonance, 1 H-NMR, size exclusion chromatography. filtrations performed pilot-size unit (ZrO 2 /TiO TiO -Al O support, 0.08 m ). To able operate without quick fouling, most parameter have high liquid over membrane, 4.7 s −1 . A good technique prefilter 50-kDa using bar pressure 5-kDa 5-bar pressure. With these settings, flux filter became 60–80 30–40 L −2 h membrane.