Extraction and purification of low molecular weight glutenin subunits using size exclusion chromatography
نویسندگان
چکیده
منابع مشابه
Allelic analysis of low molecular weight glutenin subunits using 2-DGE in Korean wheat cultivars
Two-dimensional gel electrophoresis (2-DGE) was used as a complement to SDS-PAGE to determine the allelic compositions of LMW-GS in 32 Korean wheat cultivars. Protein patterns generated by 2-DGE from each cultivar were compared to patterns from standard wheat cultivars for each allele. At the Glu-A3 locus, thirteen c, twelve d, three e (null), two g and two new alleles were identified. At the G...
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The present paper describes a method for the identification of intact high molecular weight glutenin subunits (HMW-GS), the quality determining proteins from the wheat storage proteome. The method includes isolation of HMW-GS from wheat flour, further separation of HMW-GS by reversed-phase high-performance liquid chromatography (RP-HPLC), and their subsequent molecular identification with elect...
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in this study, 110 samples of bread wheat, aegilops crassa, ae. cylindrica and ae. tauschii were investigated to identify low-molecular-weight glutenin subunits. overall, the four alleles in term of the analysis of sds-page gels were detected. the ʻaʼ allele with 45% has the greatest frequency, and after that, ʻbʼ, ʻcʼ and ʻdʼ alleles were with 38.1%, 11.8% and 9.1% frequencies, respectively; h...
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The evaluation of weight average molecular weight (Mw) and molecular weight distribution represents one of the most controversial aspects concerning the characterization of low molecular weight heparins (LMWHs). As the most commonly used method for the measurement of such parameters is high performance size exclusion chromatography (HP-SEC), the soundness of results mainly depends on the approp...
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A major problem in the measurement of serum bile acids is their quantitative extraction from the high molecular protein matrix. In our hands, the standard techniques of adsorption and reversed-phase chromatography have yielded incomplete recovery for different bile acids (33-93%) and poor reproducibility. In contrast, with the novel extraction procedure of size exclusion chromatography, recover...
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ژورنال
عنوان ژورنال: Journal of Food Science and Technology
سال: 2019
ISSN: 0022-1155,0975-8402
DOI: 10.1007/s13197-018-03560-1