Expresso® CMV system: effortless mammalian expression cloning
نویسندگان
چکیده
منابع مشابه
In silico cloning and bioinformatics study of Brucella melitensis Omp31 antigen in different mammalian expression vectors
Brucella melitensis, as a pathogenic gram-negative intracellular bacterium, causes brucellosis in animals and humans. According to literature, the B. melitensis outer membrane protein 31 (Omp31) is considered as an important vaccine candidate against brucellosis. The aim of the current study was to compare three different expression constructs containing B. melitensis Omp31 antigen using bioinf...
متن کاملGene cloning and expression of soluble thrombopoietin functional domain by harnessing Rosetta-gami expression system
Background: Thrombopoietin (TPO) is an important cytokine that has a critical role in regulating hematopoietic stem cells (HSCs) proliferation and megakaryocyte differentiation. Because of scares amount of this protein in human plasma, in many biotechnological centers around the world, recombinant production of this protein has been carried out. This study was aiming to gene cloning and express...
متن کاملCloning and Expression of Simian Rotavirus Spike Protein (VP4) in Insect Cells by Baculovirus Expression System
Background: VP4 protein is as spikes on rotavirus outer capsid shell which is responsible for virus attachment to the host. VP4 induces production of neutralizing antibodies which could be used for serotyping of different isolates. Methods: Simian rotavirus SA11 gene 4 cDNA was cloned into a cloning plasmid pDONRTM by recombination reaction using clonase II enzyme mix. The resulting clone was c...
متن کاملMammalian Cloning
On March 31st, 1978, J.B. Lippincott, a reputable New York publisher, rushed into print a remarkable and unique book. Entitled “In His Image”, this book was authored by a 34 year-old freelance science writer named David Rorvik, and provided the disturbing details of how he had helped an eccentric multimillionaire clone himself. The book centred round the desires of a very wealthy individual (co...
متن کاملA Versatile System for USER Cloning-Based Assembly of Expression Vectors for Mammalian Cell Engineering
A new versatile mammalian vector system for protein production, cell biology analyses, and cell factory engineering was developed. The vector system applies the ligation-free uracil-excision based technique--USER cloning--to rapidly construct mammalian expression vectors of multiple DNA fragments and with maximum flexibility, both for choice of vector backbone and cargo. The vector system inclu...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Nature Methods
سال: 2012
ISSN: 1548-7091,1548-7105
DOI: 10.1038/nmeth.f.356