Expression profile ofPLCζ,PAWP,andTR-KITin association with fertilization potential, embryo development, and pregnancy outcomes in globozoospermic candidates for intra-cytoplasmic sperm injection and artificial oocyte activation

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P-56: Artificial Oocyte Activation Alone Intra Cytoplasmic Sperm Injection in Men with Severe Semen Parameters

Background Failed fertilization occurs 1-3% in the infertile couples. Failure of oocyte activation (OA) is mainly attributed to failed fertilization. ICSI alone artificial oocyte activation (AOA) is only treatment approach for these individuals. Three procedures are introduced for AOA: chemical, mechanical and electrical. Most of the clinical Centers have commonly used ionomycin for activation ...

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relationship between potential sperm factors involved in oocyte activation and sperm dna fragmentation with intra-cytoplasmic sperm injection clinical outcomes

objective: the present study aimed to simultaneously evaluate the association between expression of three potential factors [post-acrosomal sheath ww domain-binding protein (pawp), phospholipase cζ (plcζ), and truncated form of the kit receptor (tr-kit)] as candidates of oocyte activation with fertilization rate and early embryonic development. materials and methods: in this experimental study,...

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Relationship between Potential Sperm Factors Involved in Oocyte Activation and Sperm DNA Fragmentation with Intra-Cytoplasmic Sperm Injection Clinical Outcomes

OBJECTIVE The present study aimed to simultaneously evaluate the association between expression of three potential factors [post-acrosomal sheath WW domain-binding protein (PAWP), phospholipase Cζ (PLCζ), and truncated form of the kit receptor (TR-KIT)] as candidates of oocyte activation with fertilization rate and early embryonic development. MATERIALS AND METHODS In this experimental study,...

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Pregnancy outcome after intracytoplasmic sperm injection with strontium oocyte activation in a globozoospermic patient.

Dear Editor, I am Dr Xiao-Yu Yang, from the Center of Clinical Reproductive Medicine in the First Affiliated Hospital at the NanJing Medical University, Nanjing, China. We present here a case report of a globozoospermic patient whose partner became pregnant after intracytoplasmic sperm injection (ICSI) with assisted oocyte activation (AOA). Globozoospermia is characterized by the presence of 10...

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Artificial oocyte activation in intracytoplasmic sperm injection cycles using testicular sperm in human in vitro fertilization

OBJECTIVE Artificial oocyte activation (AOA) is an effective method to avoid total fertilization failure in human in vitro fertilization-embryo transfer (IVF-ET) cycles. AOA performed using a calcium ionophore can induce calcium oscillation in oocytes and initiate the fertilization process. We evaluated the usefulness of AOA with a calcium ionophore in cases of total fertilization failure in pr...

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ژورنال

عنوان ژورنال: Andrology

سال: 2016

ISSN: 2047-2919

DOI: 10.1111/andr.12179